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KMID : 0613820090190010101
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Journal of Life Science
2009 Volume.19 No. 1 p.101 ~ p.110
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Intracullular Functions of the Gene in the Fission Yeast, Schizosaccharomyces pombe
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Shin Sang-Min
Cha Jae-young
Ha Se-Eun
Shim Seon-Mi
Kim Hyoung-do
Lee Jung-Sup
Park Jong-Kun
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Abstract
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The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel (mitosis associated protein) gene, a homolog of human SMARCAD1 was isolated and characterized from a fission yeast Schizosaccharomyces pombe (S. pombe) using gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 922 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that an SNF2 domain is located, which is involved in the chromosome remodeling. The quantitative analysis of the transcript against showed that the expression level of is high before septum formation in S. pombe. When null mutant cells were grown at 27 and , the cytokinesis of null mutant was greatly delayed and a large number of multi-septate and mis-segregated cells were produced. In addition, the number of multi-septate cells significantly increased. When cells were cultured in YES rich medium to increase proliferation, the abnormal phenotypes null mutant dramatically increased. These phenotypes could be rescued by an over-expression of the mast gene. The Mas2 protein localized in the nuclei of S. pombe, as evidenced by Mas2-EGFP signals. These results suggest that the is homologous to human SMARCAD1 gene and involved in septum formation and chromosome remodeling control.
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KEYWORD
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Schizosaccharomyces pombe, Mas2-EGFP, , SMARCAD1
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